Biosynthesis, characterization and antibacterial activity of bio silver nanoparticles synthesized by Bacillus algicola strain UMP 1.1

Abstract

Bio silver nanoparticles have been used as antimicrobial agents, bioremediation of waste material, effluent detoxification, decolorization of dyes, pesticides degradation and plant growth and development. In present investigation, in vitro synthesis of bio silver nanoparticles by Bacillus algicola strain UMP 1.1 was carried out by using various optimization parameters. Optimum condition parameters for maximum synthesis of bio silver nanoparticles was selected which included tryptone yeast broth containing 3g/l tryptone, 3g/l yeast extract, 30mg/l CuSO4 and 2.0mM AgNO3. Tryptone Yeast broth (pH; 8.0) using optimum condition was used for in vitro synthesis of bio silver nanoparticles at 35°C for 24 hrs of incubation. Nanoparticles synthesis indicated by change in colour from pale yellow to brown red was confirmed by UV-Vis spectroscopy with maximum absorbance peak obtained at 420nm. Characterization of bio silver nanoparticles was carried out using FTIR, XRD, DLS and SEM. These were tested for antibacterial activity against three bacterial plant pathogens.