Synthesis and characterization of Chitosan / TPP encapsulated curcumin nanoparticles and its antibacterial efficacy against colon bacteria

Abstract

The present study was designed to encapsulate curcumin by nanocoating of biodegradable polymer chitosan to enhance its stability and bioavailability and to compare its antibacterial efficacy with standard drug vancomycin against colon bacteria. Chitosan / TPP layer capsule was obtained by ionic gelation method through electrostatic interaction by mixing of equimolar concentration of both the compounds in which a known amount of curcumin was incorporated. The resulting nanoparticles were characterized by various instrumental techniques. Size of Chitosan / TPP protected curcumin nanoparticles were obtained between 135.2 nm and 72 nm by particle size analyzer and TEM analysis respectively. Morphology of nanoparticles was found to be spherical in shape by optical and fluorescence microscopes. Stability of the nanoparticles was obtained by zeta potential measurement as +12.9 mV. Antibacterial efficacy was evaluated by Antibiotic sensitivity test (ABST) by measuring the zone of inhibition scale and MIC value which was recorded as 12.5 μg / ml and 20 μg / ml for both Gram positive S. aureus and Gram negative E. Coli bacteria respectively.