Standardized the protocol for In-vitro regeneration of Bacopa monnierri (L.): An endangered medicinal plant

Abstract

Bacopa monnnieri is an herbaceous plant belongs to Scrophulariaceae family. Commonly found in India, Nepal, Sri Lanka, China, Taiwan, and Vietnam, and is also found in Florida, Hawaii and other Southern states of the USA.An experiment were conducted for standardization of in-vitro propagation techniques of Bacopa monnieri (L.), a medicinally herbs of India. Quality planting material is major factor for any economically important medicinal plants and micropropagation technique may play important role. Healthy nodal and axillary bud of the herb were used as explant with basic MS medium for shoot initiation of explant BAP (0.5 to 3 mg/l) was used. While concentration of BAP (0.5 to 3 mg/l), IAA (0.5 to 2 mg/l), NAA (0.5 to 2 mg/l) and KIN (0.5 to 2 mg/l) was used for multiplication. MS ½ strength medium with IBA (1-2 mg/l) used for rooting of plantlets. Maximum mean number of initiated plantlets 3 with shoot length 8 cm were found in MS medium treated with 2 mg BAP, while maximum mean number of multiplied plantlets 15 with shoot length 9 cm were found in MS medium treated with 1 mg BAP + 0.5 mg IAA. Maximum mean number of roots 12 with mean length 3 cmwas observed on half strength medium with 2 mg IBA. The rooted plantlets were successfully hardened in 1:1:1 ratio of sand: soil: vermicompost and successfully establishment in soil. Then fully grown plants were harden to soil by mixing soil and coco peat in ratio 2:1.