Effect of drip fertigation on endotoxin expression in intra hirsutum Bt Cotton

Abstract

Endotoxin expression in leaf of cotton genotype First Class BG-II was studied, sown under different irrigation and fertigation levels in medium deep black soil at Agricultural Research Station Dharwad, Karnataka during kharif season of 2015-16. Results revealed that expression of Cry 1Ac and Cry 2Ab protein content in leaf was non-significant at 60, 90, 120 and 150 DAS (Cry 1Ac 2.70, 1.77, 1.29 and 0.83µg g-1fresh weight and Cry 2Ab 47.66, 30.66, 25.14 and 15.14 µg g-1fresh weight, respectively) in different irrigation levels, expect at 45 DAS. Fertigation with 100 per cent recommended (RD) N & K in six equal splits recorded significantly higher protein content in leaves at 45, 60, 90 and 120 DAS (Cry 1Ac 3.33, 2.84, 2.21 and 1.33µg g-1fresh weight and Cry 2Ab 62.22, 49.32, 32.32 and 27.32) and was non-significant at 150 DAS. Cry 1Ac and Cry 2Ab protein content (3.24 and 59.33µg g-1fresh weight) were significantly higher at 45 DAS in C1 (Drip irrigation at 1.0 Etc + 100% RD N & K in 4 splits through soil (25% each as basal and at 30, 60 & 90 DAS) and no significant difference was observed for Cry 1Ac and Cry 2Ab protein content in C2 (Furrow Irrigation at 0.8 IW/CPE ratio +100% RD N & K in 4 splits through soil (25% each as basal and at 30, 60 & 90 DAS).