In vitro effect of botanicals against rice root knot nematode Meloidogyne graminicola

Abstract

This research was conducted to find out the effect of aqueous extracts of different Botanicals against the rice root-knot nematode, Meloidogyne graminicola in laboratory and Screen house conditions at CCSHAU, Hisar. Aqueous extracts from plant leaves were screened for nematode mortality against second stage juveniles (J2) and egg hatchability of M. graminicola. The nematode juveniles and eggs were exposed to 48h and 10 days to different dilutions (100%, 50% and 25%) of botanical extracts respectively. All the treatments significantly increased the percentage of mortality at 48 h. of exposure in aqueous extracts concentration (100%, 50% and 25%) as compared to control (Nematode alone). At 48 h. (100% concentration) the maximum mortality percent was found in Neem (Azadirachta indica) 89.00%, whereas in egg hatching experiment with the dilutions as 1:10, 1:20, 1:30, 1:40 and 1:50 were prepared by diluting stock solution. The Neem (Azadirachta indica) at 1:20 (v/v) dilution significantly reduced the egg hatchability and it was followed by Cauliflower (Brassica oleracea var. botrytis) and Cabbage (Brassica oleracea var. capitata).