VP6 gene based RT-PCR for detection of rotavirus associated with diarrhea in bovine calves

Abstract

The present study was undertaken on VP6 gene based detection of Rotavirus in feces of diarrheic animals. Bovine fecal samples of cattle calves (54) between the age group of 0-3 months were collected from diarrheic animals from organized and unorganized farms in and around Nagpur. Of them 11 (20.37%) out of 54 samples were found positive for bovine rotavirus by latex agglutination test. The positive sample of calf was subjected to VP6 gene based Reverse Transcriptase polymerase chain reaction which yielded amplicon of 379 bp RT-PCR targeting and confirmed Group A rotavirus. VP6 gene base RT-PCR study confirmed the prevalence of group A Rotavirus in animals and can be used as a sensitive and specific assay for the rapid detection of group A Rotavirus in fecal samples.