Anti-mycobacterial activity of Acacia nilotica leaves extracts against Mycobacterium smegmatis

Abstract

The present investigation was carried out to determine the anti-mycobacterial activity of hexane, chloroform, butanol, ethanol, aqueous extracts and active acetone fraction of Acacia nilotica leaves by disc diffusion, minimum inhibitory concentration, and minimum bactericidal concentration tests against Mycobacterium smegmatis. In disc diffusion method, the active acetone fraction of A. nilotica leaves showed best inhibiting activity compared to other extracts. It showed diameter of 52 mm zone of inhibition at 150 mg/ml concentration. Whereas the standard dapsone showed 67 mm zone of inhibition at 50 mg/ml. In MIC test, among other extracts, the active acetone extract showed lowest MIC at 0.15 mg/ml. In MBC test also the active acetone extract comparatively showed minimal MBC at 0.28 mg/ml.