Detection of FFA content of coconut milk by using screen-printed electrode

Abstract

Coconut milk is an aqueous solution which is extracted from coconut meat from pressing or squeezing. The quality attributes of coconut milk are profoundly affected by many factors, and coconut milk is highly susceptible to chemical and biochemical spoilage like lipid oxidation. The quality of coconut milk is usually found out by FFA (% of lauric acid) content in it, but it requires a lot of solvents and complicated procedure. The screen-printed electrode is made of flexible or rigid substances that can be printed on a flexible PET film or substances. Screen-printed electrode biosensor was used to analyse the Triglyceride content and to correlate with FFA content to check the quality of coconut milk. The triglyceride content and FFA content was analysed for the 5-hour interval for two days and found that FFA content was increasing from 0.448 to 1.455 at the end of 45 hours and triglyceride content was decreasing from 1.18 to 0.36mM. The validation of the developed sensor was showing good results with the UHPLC method with a coefficient of determination (R2=0.96). The performance valuation of the developed biosensor was also evaluated.