Genetic relationship analysis of hybrid green catfish (Hemibagrus nemurus) with striped catfish (Pangasianodon hypophthalmus) by random amplified polymorphic Dna (Rapd) method

Abstract

This research conducted to determine the genetic variation on male green catfish (Hemibagrus nemurus), female striped catfish (Pangasianodon hypophthalmus) and genetic relationship of hybrid to each of parent. The research method was an explorative experiment. This research was conducted using striped female striped catfish, male green catfish, and the hybrid of striped female catfish and male green catfish. Genetic variation analysis was performed using the RAPD-PCR technique using primary OPA-2 and OPA-3 methods.However, the result of DNA amplification with OPA-3 was better than OPA-2. Therefore, further analysis was carried out using data from OPA-3. The genetic relationship was analyzed based on the similarity index which calculated by Numerical Taxonomy and Multivariate Analysis System (NTSYS) program. The result showed that the similarity index between hybrid and female striped catfish was 82%, while the similarity index between female striped catfish and male green catfish was 31%. Thereby, the inheritance of hybrid comes more from female striped catfish.