Molecular cloning of 1-396 BP of goat Vitronectin (VTN)

Abstract

The extracellular matrix protein Vitronectin has immense importance in cell spreading, migration and adhesion. The roles are not confined to these rather it also regulates complement cascade and adhesion of bacterial pathogens. The molecule possesses various domains which renders it to be multifunctional. Goat vitronectin significantly differs from human vitronectin in possessing extra RGD sequences and absence of any signal sequence. In this study initial 396bp of goat VTN was cloned into a prokaryotic expression vector. The clones were further confirmed by colony PCR and gel retardation assays.