Ameliorative effect of free radical scavenger aminoguanidine hemisulfate on amikacin induced biochemical alteration in Wistar rats

Abstract

The present study was aimed to investigate the biochemcal alterations induced by subacute administration of amikacin and the effect of aminoguanidine hemisulfate alone and their combination in wistar rats of either sex. Twenty-four healthy wistar rats were randomly divided into 4 groups (I, II, III and IV) and each group comprises of 6 animals. Group-I served as control to which normal saline was administered. For sub-acute study rats of group-II and group-III were treated with amikacin (15mg/Kg BW) and aminoguanidine hemisulfate (20mg/Kg BW) daily for 28 days intra-peritoneally, respectively. In group-IV rats, amikacin and aminoguanidine hemisulfate were co-administered at their respective doses. A significant increase in the biochemical-parameters such as ALT, plasma-creatinine, total bilirubin, plasma uric acid and plasma urea nitrogen were found as compared to control group after 28 days of intra-peritoneal administration of amikacin. However, a significant decrease in plasma albumin was found as compared to control group.