Studies of pathogenic variability, Mycelial Compatibility and Incompatibility Groups of Fusarium Oxysporum F. Sp. Ciceri Isolates

Abstract

Studies of Pathogenic Variability, Mycelial Compatibility and Incompatibility Groups of Fusarium Oxysporum F. Sp. Ciceri Isolates Laboratory studies of fifteen isolates of Fusarium oxysporum f. sp. ciceri, obtained from different locations were categorized into various groups based on cultural characters and pathogenic variability. Results of this study shows that the isolates varied in colony colour (white, dirty white and milky white), type of growth (fluffy and compact), growth rate (fast and slow), Pathogenecity (weakly pathogenic, moderately pathogenic, highly pathogenic). Based on cultural characters and relative Pathogenecity of 15 isolates of Foc studied were finally characterized into 5 groups. Five isolates viz., 16, 60, 80, 105 and 88 were found highly pathogenic with medium growth i.e., 70-79 mm, while 3 isolates exhibited fast growth and highly pathogenic too. Seven isolates belonging to moderately pathogenic group which are either medium or slow growing (<70mm). Mycelial compatibility among the isolates was also observed and out of 105 combinations only 72 combinations (68.5%) showed compatible reaction and 22 (25.9%) whereas vegetative incompatibility groups were identified. Almost 31.42 per cent combinations i.e. 33 showed antagonistic reaction. Microscopic observations on mycelial interaction revealed protoplast lysis, vacuole formation, hyphal thickness and hyphal lysis.