Direct organogenesis and microrhizome production in ginger (Zingiber officinale Rosc.)

Abstract

This study developed an effective protocol for in vitro shoot multiplication and microrhizome induction in ginger (Zingiber officinale). In vitro culture was established using sprouting shoot bud explants of ginger rhizome. A concentration of 1.0 mg/L BA and 0.5 mg/L NAA found to be optimum for shoot induction. Different plant growth regulators, photoperiod exposure level and sucrose concentration were investigated for microrhizome induction. The optimal response was observed in the MS medium containing 0.5 mg/L BA and 0.5 mg/L IAA. A healthy and maximum microrhizome production was obtained in the MS medium consisted of 8% sucrose under 8 hour photoperiod. This study can be used to develop protocols for mass production of pathogen-free microrhizome and conservation of ginger.