Coat protein based characterization of Mungbean yellow mosaic virus in Tamil Nadu

Abstract

Yellow mosaic disease caused by Begomovirus (Mungbean yellow mosaic virus (MYMV) or Mungbean yellow mosaic India virus (MYMIV)) has become an important constraint in mungbean production. Survey was conducted on five different mungbean growing localities in Coimbatore district of Tamil Nadu to explore the strain MYMV or MYMIV. PCR based characterization of strains (MYMV /MYMIV) were carried out using their gene specific primers respectively. Distinct viral gene specific PCR product corresponding to CP ~703 bp and MP ~893 bp was obtained for MYMV and no amplification was seen on MYMIV. The sample was further confirmed by CP gene sequencing. The obtained sequence was compared with the other selected begomovirus sequences from the NCBI blast database. Results were found to show high sequence identity to MYMV (100%). Phylogenetic analysis of CP gene sequence of our isolate with selected begomoviruses showed clustered with isolate of MYMV with more homology. While in contrast lesser homology was seen among MYMIV isolate, confirming that the begomovirus causing yellow mosaic disease of mungbean is explored to be as strain of MYMV and not as MYMIV among our geographical location isolates.