Biochemical and molecular analysis of wild rice and maize (non-host) against R. solani

Abstract

Sheath blight caused by Rhizoctonia solani, is a serious disease of rice crop grown worldwide. In rice, (O. sativa L.)], complete genetic resistance does not exist. For the identification of possible gene(s) involved in defense against this pathogen during infection, wild rice accession Oryza rufipogan, and nonhost maize cv. Kanchan has been showed moderately resistance. Total RNA and protein has been isolated from the control and inoculated plants leaves. Isolated mRNA was converted into cDNA and differentially expressed proteins were studied with RGA primers. The biochemical processes underlying the expression of resistance to R. solani infection were investigated and compared in wild rice, and non-host maize. Some important resistance gene analogue fragments and proteins were showed higher expression in wild rice (Oryza rufipogan) in comparison to maize cv. Kanchan. These induce RGA and biochemical signals will be further used for the investigation of resistance against sheath blight pathogen.