Molecular characterization of wheat genotypes using eSSR Markers

Abstract

Wheat is an important Rabi Crop, which is grown between October and December and harvested between April and May. Globally, wheat is the leading source of protein than maize or rice. A study was undertaken to examine the genetic diversity of 20 wheat (Triticum aestivum L.) using designed EST-SSR markers. The similarity coefficient value for all the 20 genotypes ranged from 0.23 to 0.63. The minimum similarity exhibited by genotype PBW550 and DBW17. Whereas, the maximum similarity was shown by genotype DBW16 and RAJ4246. The pair wise genetic similarities among all pairs of samples were estimated and subjected to cluster analysis. The UPGMA based clusters shows that all the genotypes are interlinked with each other and exhibited high genetic similarity. The UPGMA based clustering of 20 wheat genotypes grouped the wheat genotypes into three distinct clusters at 70% similarity coefficient.