Phytochemical analysis of secondary metabolites on Pogostemon auricularis (L.) Hassk. and Anisomeles malabarica (L.) R. BR. ex Sims

Abstract

The present study was made an attempt to compare the preliminary phytochemical constituents of the leaf extracts of two extreme environments such as: hydrophytic (Pogostemon auricularis) and xeric (Anisomeles malabarica) plants. The dried leaf sample was extracted with three different solvents such as petroleum ether, methanol and water. The quantitative analysis of the total phenolics, flavonoids, tannin and alkaloids were assayed using the standard protocols. The maximum yield of extract (28% W/W) was record in P. auricularis of methanol extract. But, the A. malabarica showed maximum (20% W/W) extraction in methanol extract. Of the different solvent extractions the highest concentration secondary metabolite was identified with the total phenolic content in methanol extract of P. auricularis (54.59±0.5 (mg GAE/g extract) and A. malabarica of (48.28±0.5 mg GAE/g extract) respectively. The present investigation found that the remarkable variation in flavonoids, tannin and alkaloids. It may pave a new way to identify the novel secondary metabolites.