Production and characterization of PPBS from Brevundimonas aurantiaca KY231210 and Enterobacter cloacae KY231211

Abstract

The aim of present study was production of biosurfactant from selected microorganisms i.e. Brevundimonas aurantiaca KY231210 and Enterobacter cloacae KY231211 and after extraction characterization of PPBS via oil displacement assay, blood haemolysis assay, emulsification index with vegetable oils in different incubation (0, 24, 48, 72 and 96h) and estimation of carbohydrate, protein and lipid content. Mustard oil and sunflower oil were used for carbon source for production. Higher biosurfactant production was obtained with mustard oil. Brevundimonas aurantiaca KY231210 proved as better producer of biosurfactant production than Enterobacter cloacae KY231211 and recorded 4.31g/l yield. PPBS was obtained by solvent extraction and drying process. PPBS of Brevundimonas aurantiaca KY231210 showed highest zone of oil displacement than PPBS of Enterobacter cloacae KY231211 in both concentration (10µl and 20µl). PPBS of both bacterial cultures showed highest hemolytic zone on blood agar plates than culture and crude. PPBS of Enterobacter cloacae KY231211 showed β heamolysis whereas PPBS of Brevundimonas aurantiaca KY231210 showed α haemolysis. Emulsification index was decreased after each incubation. PPBS of Enterobacter cloacae KY231211 showed highest Emulsification index with soybean oil in 0h incubation while sunflower oil gave highest Emulsification index with PPBS of Brevundimonas aurantiaca KY231210. Presence of carbohydrate, protein and lipid was recorded in PPBS. PPBS of Enterobacter cloacae KY231211 showed higher amount of carbohydrate while protein content was higher in PPBS of Brevundimonas aurantiaca KY231210.