Role of Nano-curcumin on carbon tetrachloride (CCl4) induced hepatotoxicity in rats

Abstract

To investigate the hepatoprotective effect of nanocurcumin on carbon tetrachloride induced hepatotoxicity, 48 rats were divided into eight groups consisting six animals each. Hepato-toxicity was induced using CCl4 at the dose of 2 ml/kg body weight intraperitoneally twice weekly. Nanocurcumin prepared by evaporative-precipitation of nanosuspension was administered at the dose of 40, 80 and 160 mg/kg body weight orally daily for 28 days. The protective effect of nanocurcumin was compared with curcumin and turmeric (160 mg/kg body weight). Silymarin was used as standard. Results revealed that CCl4 elevated LDH and liver enzymes (AST, ALT, ALP) activities, bilirubin and uric acid blood levels, and decreased total protein, albumin, and globulin. Nanocurcumin significantly decreased liver enzymes (AST, ALT, ALP) and LDH activities, bilirubin and uric acid, and increased levels of total protein, albumin, and globulin in a dose-dependent manner. It elucidated the hepatoprotective effect of nanocurcumin on carbon tetrachloride induced hepatic-toxicity.