Evaluation of neuroprotective activity of isolated fraction from Biophytum sensitivum on #945;-(hydroxymethyl) Benzeneacetic acid 9-methyl-3-oxa-9-azatricyclo non-7-yl ester induced dementia in rats

Abstract

Objective: To isolation of fractions, estimation of In Vitro anti oxidant and Neuroprotective effects of Fraction A from Methanolic extract of Biophytum sensitivumon α-(hydroxymethyl) benzeneacetic acid 9-methyl-3-oxa-9-azatricyclo non-7-yl ester induced Dementia. Methods: In this study in vitro antioxidant activity is estimated by DPPH assay method. Neuroprotective activity is estimated by α-(hydroxymethyl) benzeneacetic acid 9-methyl-3-oxa-9-azatricyclo non-7-yl ester induced dementia method. In neuroprotective method behavioral parameters were checked by Escape latency time (Sec) by using Morris water maze, Active avoidance testing by using Elevated plus maze and nuerochemicals like glutathione, catalase, and Cholinesterase were estimated. Results: In α-(hydroxymethyl) benzeneacetic acid 9-methyl-3-oxa-9-azatricyclo non-7-yl ester induced dementia increased escape latency time, active avoidance and neurochemicals like glutathione, catalase and Cholinesterase and lipid peroxidase levels increased. Whereas Standard and Fraction A treated animals decreased in escape latency time, active avoidance and neurochemicals like glutathione, catalase are increased and Cholinesterase and lipid peroxidase levels were increased. For in vitro antioxidant we calculate the IC 50 value. IC 50 value of ascorbic acid is 7.501 and IC 50 value of HEMBS Fraction A is 8.315 Conclusion:The present study clearly demonstrates that HEMBS Fraction A (50 and 100 mg/kg) significantly attenuate α-(hydroxymethyl) benzeneacetic acid 9-methyl-3-oxa-9-azatricyclo non-7-yl ester -induced dementia by improving the learning, memory, antioxidant potentiality and anti-acetylcholinesterase activity. Therefore, this Fraction A can be a potential novel therapeutic strategy for controlling neurodegenerative dementia especially AD. Yet, advance studies are needed to characterize the active compound(s) and expose the possible mechanism of action.