Isolation and identification of antagonistic Lactobacillus acidophilus from curd against MDR enteric pathogens

Abstract

The present study is directed towards isolation and identification of antagonistic Lactobacillus acidophilus obtained by curd collected from local market of Prayagraj. After careful examination of cultural, morphological and biochemical characteristics, six strains of Lactobacillus acidophilus namely LA1, LA2, LA3, LA4, LA5 and LA6 were isolated. Antimicrobial activity of these six strains were evaluated by agar well diffusion method against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Salmonella typhi. Cell free supernatant obtained from the isolated strains exhibited maximum antimicrobial activity against selected pathogens and the result reveals that strains of Lactobacillus acidophilus have a good antagonistic property and thus revealing that curd is safe and beneficial for consumption.