In vitro and In vivo efficacy of different chemicals against Bacterial leaf blight disease of rice caused by Xanthomonas oryzae Pv. oryzae

Abstract

Rice (Oryza sativa L.) belongs to family Poaceae (Gramineae) and widely cultivated in the India. The maximum losses of rice crop by Bacterial leaf blight stands one of the important disease of rice caused by Xanthomonas oryzae Pv. oryzae and which reduces the yield upto 50% in the world. In India yield losses due to this disease is up to 81.3%. In present investigation, different chemicals were tested under in vitro and in vivo condition against Bacterial leaf blight disease of rice causing by Xanthomonas oryzae pv. oryzae. Combination of [Azoxystrobin 25% + Difenoconazole 12.5%] @ 300 ppm + Streptocycline @ 200 ppm was found most effective with inhibition 31.62% and was at par with [Mancozeb (12%) + Carbendazim (63%)] @ 500 ppm + Streptocycline @ 200 ppm 30.48% inhibition against Xanthomonas oryzae pv. oryzae. Fungicide alone tested at different concentration and found that [Azoxystrobin 25% + Difenoconazole 12.5%] @ 300 ppm was found most effective with inhibition 21.66% and was at par with [Mancozeb (12%) + Carbendazim (63%)] @ 500 ppm i.e. 19.33% inhibition, whereas Streptocycline @ 200 ppm showed 23.49% inhibition against Bacterial leaf blight disease of rice in vitro condition. Under in vivo condition five fungicides and two antibiotics were tested and found that the combination of Copper oxychloride @ 0.3% + Streptocycline @ 0.005% showed minimum disease incidence with 39.64%. Therefore, it could be useful to the farmers to manage the Bacterial leaf blight disease of rice by application of Copper oxychloride @ 0.3% + Streptocycline @ 0.005%.