Validated high-performance thin-layer chromatography method for determination of Piperine in herbal extracts and pharmaceutical dosage form

Abstract

Herbal medicines widely used in health-care in both developed as well as developing nations. Herbals are usually considered harmless and ever supplementary being consumed by people devoid of prescription. Though, some of them leads to health troubles, some are not effective and some might be interact with supplementary drugs. Consequently, standardization of herbal formulations is essential in order to estimate the quality of drugs, based on the concentration of their active principles. In the present study, Shatavari based Galactogogue, herbal formulation manufactured by Fredun Healthcare Pvt. Ltd., Mumbai is used for standardization. The HPTLC method was validated according to ICH guidelines and shown to be specific, linear, repeatable and accurate, within the established ranges. Statistical analysis proves that the method is reproducible and selective. This method can be used for the quantitative determination of piperine in herbal extract and its formulations.