GC-MS analysis and antibacterial activity of Cleome rutidosperma, Cleome gynandra and Cleome viscosa seed extracts: A comparative study

Abstract

Phytocomponents present in the chloroform seed extracts of Cleome rutidosperma, Cleome gynandra and Cleome viscosa species were analayzed by Gas Chromatography – Mass Spectrometry method. The air dried seeds were powdered and subjected to chloroform and methanol solvent extraction. Then each of these extracts was further subjected to Gas Chromatography-Mass spectrometry and its antibacterial activity analyzed. Qualitative determination of the different biologically active compounds from the crude extracts of Cleome species seeds using Gas Chromatography-Mass Spectrometry revealed different types of high and low molecular weight chemical entities with varying quantities present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. The extracts were found to possess significant dose dependent antibacterial activity against both Gram positive and Gram negative bacteria. This study confirmed antibacterial property of the plants studied and its potential as a source of plant based drug.