Molecular characterization of the Tobacco streak virus infecting peanut

Abstract

Tobacco streak virus (TSV) is belongs to the family Bromoviridae and genus Ilarvirus; fast increasing plant pathogen infecting more than 200 plant species belonging to 30 dicotyledonous and monocotyledonous plant species and transmitted by the Arthropod vector thrips. Peanut is very prone to Tobacco streak virus; causing peanut stem necrosis leads to huge economic losses. In present study we have collected the peanut plant showing characteristics symptom of the TSV; diagnosed through Direct antigen coating- Enzyme linked imminosorbent assay (DAC-ELISA) as well as Dot immunobinding assay. Coat protein gene of the TSV was amplified, cloned and sequenced. The identified coat protein gene sequenced was compared with the sequence available in NCBI data base. The identified peanut strain of TSV showed 99.78% homology to the Kurnool peanut strain of TSV (Accession no AY50508).