Effect of ethnomedicinal plants on osteoblast proliferation using cell line based assay method

Abstract

Osteoporosis reduced bone strength and it is responsible for bone fractures. Worldwide, approximately one in three women and one in five men over the age of 50 will suffer an osteoporotic fracture in their remaining lifetime. Since many decades, herbal plants are utilized to increase bone health, however many of them are not scientifically evaluated. We selected herbal plants on the basis of their ethnobotanical use of scientific evidence that suggests a potential efficacy in the treatment of bone-loss diseases. This study was aimed to evaluate the cell proliferation activity of selected ethnomedicinal plants on MG 63 cell line. Aqueous and ethanolic extracts of Aspargus racemosus (Ar), Berberis aristata (Ba), Emblica officinalis (Eo), Hemidesmus indicus (Hi) and Nigella sativa (Ns) were screened for osteoblast cell proliferation study using MTT assay method. Three different ranges of concentration were selected for each extracts and percentage proliferation was analyzed using MTT assay. In conclusion, Ns have a highest cell proliferation capacity amongst selected plant. Ar and Ba cause inhibition effect on cell. Wherever, Eo and Hi cause moderate stimulatory effect on MG 63 cell line.