Molecular Identification of the enterotoxin B gene from Staphylococcus aureus and the enterobactin B gene from Klebsiella pneumoniae

Abstract

One hundred and five of clinical samples were collected from Central Teaching Hospital of paediatric and Medical city / Educational laboratory, period from 17/10/2016 to 23/1/2017, Klebsiella pneumoniae and Staphylococcus aureus isolates were diagnosed molecularly by using polymerase chain reaction, enterobactin B and enterotoxin B genes were amplified by using multiplex PCR. Multiplex PCR was performed for K. pneumonia and S. aureus isolates with two primers that target the ent B (enterobactin B) and entB (enterotoxin B) genes respectively. PCR was performed with the primer that targets the ent B (enterobactin B) gene, the result revealed that all eight samples of the K. pneumoniae isolates gave a band of 330 bp in size. On the other site the other primer that targets the ent B (enterotoxin B) gene, the result shows that only two isolate of S.aureus having a band of 411 bp in size. The reason leads to not all S. aureus species had enterotoxin B.