Induction genetic variability using ems and its molecular analysis using RAPD, ISSR and SSR markers in cotton

Abstract

The objective of the present work includes induction of genetic variability by chemical mutagen ethyl methane sulfonate and assessment variation in genomic DNA through RAPD, ISSR and SSR markers caused due to mutagenic effect of EMS in cotton. The two varieties of cotton viz., Gossypium hirsutum (GSHV-01/1338) and Gossypium arboreum (G.27) were subjected to different EMS concentration (0.75%, 1.5%, and 2.25%) treatment. Non treated plants were served as control. EMS @ 2.25% concentration found to be lethal in G.27. Observation indicated that as the concentration of EMS was increased, germination percentage, root length and shoot length was decreased. Further, biological effects of EMS on the total genomic DNA of the treated plants studied through RAPD, ISSR and SSR markers indicated, induced variation resulted in genetic distance to an extent of 2 per cent in G.27 and 1 per cent in GSHV (01/1338) in comparison to non-treated (control) varieties