Study on expression of PR-proteins and defense related enzyme during interaction of rice differentials with Magnaporthe oryzae Cav.

Abstract

Magnaporthe oryzae Cav. (Anamorph Pyricularia oryzae (Cooke) Sacc.) is a cause for a very serious and threatful biotic stress called rice blast. Managing the disease through PR protein was found to be an effective approach as over expression helps in antimicrobial control. An experiment was conducted using 25 rice differentials to study pathogenesis-related (PR) protein expression and activity of enzymes such as ascorbate peroxidase, guaiacol peroxidase, glutathione reductase and superoxide dismutase during pathogen attack as they are indication of defense activation. Among them, Tetep was found resistant where in HR 12 and CO 39 was found highly susceptible to rice blast. PR 11 was expressed in resistant and moderately resistant differentials but it was unnoticed in highly susceptible differentials (HR 12 and CO 39). Activity of above mentioned defense related enzyme was found higher after 48 hours of inoculation in prominent rice variety CO 39. Among other 24, RIL 10, a moderately resistant differential recorded maximum activity of ascorbate peroxidase and glutathione reductase. The higher reports of guaiacol peroxidase and superoxide dismutase activity was observed in IR 64 and Kanto 51 respectively (moderately resistant differentials). All four enzyme activity was lowest in susceptible to highly susceptible differentials.