Molecular screening of blast resistant gene in thirty rice (Oryza sativa) genotypes through Blast specific primers

Abstract

Rice is the first cereal genome with a complete sequence and a model crop that has important relationships with other cereal species. It has a small genome size of 430 Mb (IRGSP, 2005) which is one-tenth the size of the human genome. The complete sequence is available for both japonica and Indica genomes. Among the thirty rice genotypes, PB-2 showed the minimum disease severity at 7 DAI (1.40%) and at 14 DAI (1.50%) followed by Tetep showed the minimum disease severity at 7 DAI (1.40%) and at 14 DAI (1.50%) from tillering stages whereas, Basmati-370 showed the maximum disease severity at 7 DAI (3.90%) and at 14 DAI (3.93%) from tillering stages. The five genotypes (Ranbeer Basmati, PB-1, VB-23, BPT-5204 and B-370) which showed the absence of Blast resistance genes indicated the highest AUDPC (65-84), therefore regarded as BLAST susceptible genotypes. The five genotypes (HB-1, N-22, PR-106, Tetep and PB-2) which showed the absence of Blast resistance genes indicated the lowest AUDPC (6-12), therefore regarded as Blast resistant genotypes.