Detection and identification of seed borne myco flora of wheat (Triticum aestivum L. em. The ll.) Seed samples

Abstract

Seed borne myco flora of Wheat in two district of southern Rajasthan (Chittorgarh and Udaipur) were surveyed. A total of 80 seed samples, 40 of each district, were collected during 2015-16. Data were recorded for seed germination percentage, percent pathogen frequency and major seed borne myco flora, which were identified and quantified using the blotter and agar plate method as recommended by ISTA (International Seed Testing Association).Seed germination percentage were found minimum 82.9% in Chittorgarh district CH-6 sample and maximum 86.10% in CH-14, CH-40 (Chittorgarh), UD-14 and UD-21 (Udaipur ). Eight wheat seed borne myco flora viz., Alternaria alternata, Aspergillus flavus, A. niger, Curvularia lunata, Fusarium moniliforme, Rhizopus stolonifer, Mucor spp. and Trichoderma viride were detected and isolated from eighty seed samples by using standard blotter paper and agar plate methods. The maximum infected embryo was observed in Chittorgarh district seed samples while minimum was observed in Udaipur district seed samples.