Mutagenic sensitivity in M1 generation of three verities of soybean (Glycine max L.)

Abstract

In this study, variability was induced by gamma rays in three soybean genotypes viz. Phule Agrani (KDS-344), Phule Sangam (KDS-726) and KS-103 were selected for mutagenic treatment. Their seeds were exposed to three doses of gamma-radiation 200, 300, and 400 Gy in order to study their mutagenic effects in the treated seeds in M1 generation. The observations were recorded on germination percentage and survival percentage. The gamma ray treatment was done at Mutation Breeding Section, Department of Nuclear and Agriculture, BARC, Trombay, Mumbai. The observations on mutagenic effects in Ml generation revealed dose dependent reduction in seed germination and survival. Among the genotypes, KS-103 soybean was more sensitive for mutagenic treatments than Phule Agrani and Phule Sangam. The LD50 dose between 300-400 Gy gammas were found to be ideal for mutagenic treatments irrespective of the genotype.