Preparation of external herbal formulation for wound healing activity

Abstract

Wound healing is a tedious process involving multiple parameters. Wounds happen during trauma, sports, skin burning etc. Skin is the largest organ in terms of surface area. Skin is composed of triple helical collagen tissue which is giving strength to skin. In this experiment attempt was made to determine the wound healing activity of selected medicinal plants mainly Acorus calamus, Hibiscus rosasinenesis, Mimosa pudica which are available in our locality. The plants were extracted with solvents selected from previous research activities. The preliminary phytochemical screening was performed to know the major phytochemical in each plant extract. The creams were prepared using oleaginous base, absorption base, preservative and flavouring agent as prescribed by official books. Accelerated Stability study of six months was performed on creams and best stable cream was used for further activity. Acute toxicity study was performed to know the therapeutic dose and toxic dose of extracts. Determined Wound healing activity using major two models excision wound model and incision model, which are used to identify percentage, wound closure and tensile strength of newly synthesized skin. Further the major marker amino acid compound Hydroxyproline was determined as a supporting factor to the wound healing activity. By this study we come to conclusion that the selected medicinal plants are good wound healing herbs if prescribed in correct dosage form and appropriate formulation suitable to the better skin compatibility.