Isolation and characterization of bacterial isolates from agriculture field soil of Roorkee region

Abstract

Microorganisms present in soil plays a major role in enhancing the plant growth. In our present study soil sample was collected from the agriculture field of Quantum Global Campus, Roorkee and bacterial organisms were isolated by serial dilution technique. Well defined isolated colonies were selected and pure cultured. The isolates were named as QAF01, QAF02, QAF03, QAF04 and QAF05. Biochemical characterization of the above mentioned isolates determined that 20% of the isolates were spore formers, 60% were motile in nature, 100% were rod shapers in morphology and 60% were gram positive in reaction. Metabolism of various sugars was done at a maximum rate by the isolates QAF04 and QAF03. Based on their biochemical characterization and carbohydrate fermentation the isolates were identified to be Bacillus, Pseudomonas, Streptomycis, Azotobacter and Alcaligenes.