Simultaneous marker based standardization of in-house gel by HPTLC with gallic acid and Berberine

Abstract

The present paper deals with marker based standardization of in-house gel containing ethanol extract of dried peels of punica granatum and dried root bark of berberis aristata. The bioactive markers were gallic acid and Berberine used to develop and validate the High Performance Thin Layer Chromatographic method. The method was performed on TLC aluminum plates Precoated with silica gel 60F254. Good separation was achieved in the mobile phase composed of toluene: ethyl acetate: methanol: formic acid (3.5: 5: 1:0.5) and detected at wavelength 254nm in reflectance/absorbance mode. The developed method was well validated by different parameters mentioned in ICH guidelines such as linearity, accuracy, precision, robustness.