In-vitro screening of nematode trapping fungi against root knot nematode

Abstract

The nematode trapping fungi comprise more than 700 species found in all major taxonomic groups. The nematode trapping fungi, which infect nematodes with their hyphal traps or conidia, are more dependent on nematodes as a nutrient and considered as obligate parasites. In this study nine species of nematode trapping fungi were compared with different experiment in vitro viz. effect of temperature, effect of different media, effect of different pH, effect of carbon nitrogen sources for their growth. In all the experiment conducted in vitro and was found that the most potent nematode trapping fungi as Arthrobotrys oligospora followed by Monacrosporium eudurmatum and least growth was observed in Dactylaria brochopaga. Among these species potential of predacity and trapping structure induced by nematode trapping fungi in the presence of root knot nematode was also assessed and found Arthrobotrys oligospora killing 49% root knot nematodes (Meloidogyne incognita) and trapping structures induced by this fungi was 2+ (26-50 traps) after 96 hours. The least predacity was observed in Dactylaria brochopaga having predacity of 12% with similar number of induced trapping structures i.e. 2+ (26-50 traps) observed after 96 hours.