Screening and characterization of Aristolochia bracteolata plant extract against antibacterial activity of selected microbes

Abstract

A bioassay-guided assay of acetone extract of Aristolochia bracteolata plant leaf was carried out in order to evaluate its antibacterial activity and to identify the active compounds in this extract. Antibacterial activities of acetone extract against gram-positive and gram-negative bacteria strains were investigate by the kirby bauer agar disk diffusion method. Among the strains tested Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. Showed the highest sensitivity towards the acetone extracts and hence they are used as test organisms for the bioassay-guided fractionation. From this extract, aristolochic acid. Has been isolated and has showed the greatest antibacterial activity against both standard strain and clinical isolates of Escherichia coli with equal minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 30 and 50 𝜇g/mL. The plant leaf having bioactive compound of aristolochic acid and acetylenic acid and flavonoids. Bioactive components and functional groups present in the plant leaf essential for aristolochic acid antibacterial activity. This investigation it can conclude the Aristolochia bracteolata for alternative of treatment for bacterial infection in traditional and current medicinal uses.