Identification, validation of Comp121307 C0 Seq4 gene against leaf rust resistance gene in ILT598, ILT756 and WL711 derived from Aegilops geniculata

Abstract

The current investigation was conducted during the 2017-18 sample collection under six-time intervals 0, 12, 24, 48, 72 and 96hr. All six different time interval samples were collected from the Genetics area, school of agricultural biotechnology (SOAB) PAU Ludhiana (state university). To find out the candidate gene from a raw sequence provided by bioinformatics lab SOAB as well based on the candidate gene to check to fold expression in IL T598, IL756 and WL711 as well visual fungal biomass investigation. Result directly shown comp_121307_c0_seq4 is the candidate gene which reveals the maximum fold expression at 24hr among all six-time interval was 5.40 - ILT598, 1.22- ILT756 and WL711 simultaneously less fungal mass was observed at 24hr in ILT598, more in IL756 and more than all two in WL711. Fungal biomasses in challenged leaves of ILT598 were less compare to ILT756 and WL711 which directly correlated with host-pathogen interaction (Cheng et al. 2012).