A research on development and validation of HPTLC method for simultaneous estimation of Ofloxacin, Clotrimazole and Ornidazole in their combined dosage form

Abstract

High Performance Thin Layer Chromatography using silica gel aluminum plate 60F254 (10*10cm) as stationary Phase and Methanol: Chloroform: Ammonia (3:5:2) as Mobile Phase. The developed plates scanned densiometrically using UV 273nm wavelength. The Rf value of Ofloxacin, Clotrimazole and Ornidazole was found to be 0.49, 0.73 and 0.89 respectively. The method was validated for different validation parameter such as linearity, accuracy, precision, LOD, LOQ and robustness and the result were found to be within the acceptance limit as per the guideline of international conference on Harmonization (ICH).