Production and characterization of extracellular compounds from Pseudomonas aeruginosa and their impact on Dendrocalamus asper under in vitro stress condition

Abstract

Abiotic stress factors affecting plant growth and production around the world. In this experiment, in vitro multiplication of Dendrocalamus asper has been standardized. Pseudomonas aeruginosa producing metabolites are improved the plant immunity, altered the signaling pathway and produce stress tolerance plants. Pseudomonas aeruginosa was isolated from soil and identified using 16S rRNA gene sequencing. The pyocyanin production medium were optimized and successfully extracted their extracellular metabolites using chloroform extraction method. Bamboo was cultivated with MS medium supplement with BAP (1, 2 & 3mg/l) were maintained for mass multiplication. The media was optimized with BAP 1mg/l is produced multiplication of shoot and mass culture. To study the effects of pyocyanin on stress in bamboo (Dendrocalamus asper), the explants were treated with varying concentrations of pyocyanin (0.2, 0.4, 0.6 & 0.8mg/l) under in vitro condition. The physiological and morphological responses of the crop against pyocyanin stress has been identified. The Chlorophyll pigment present in the plant was decreased due to stress condition. Pyocyanin induced an accumulation of phytohormones in the leaves under in vitro stress condition. In pyocyanin treated plants are significantly increased the proline content. Pyocyanin and was found to be effective against abiotic stress with the help of Morphological and Physiological test.