Preliminary phytochemical screening and HPTLC method for qualitative determination of phytochemical compounds in extract of Ehretia laevis Roxb

Abstract

Background: The plant kingdom for its therapeutic use has been inadequately explored and there is a tremendous scope for the drug discovery for the human health problems. India has rich in plant wealth and also having excellent usage of medicinal plants with Ayurvedic science. Objective: The objective of this study was to carry compound class detection present in plant extracts using chromatographic and by Phytochemical screening methods. Materials and Methods: Preliminary phytochemical screening was done, physical constants were evaluated and HPTLC studies were carried out. Results: Preliminary phytochemical screening and HPTLC analysis of the extract showed the presence of Triterpenes, Tannins, Saponins, Glycosides, Phenolic compounds and flavonoids.Conclusions: It can be concluded that HPTLC analysis of extract can be used as an authentic diagnostic tool for the correct identification of secondary metabolites and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.