Green synthesis of silver nanoparticles using Hygrophila auriculata seed extract with antibacterial activities

Abstract

In the present study, biosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Hygrophila Auriculata plant seed act as a reducing and capping agent has been reported in the present work. Biosynthesized AgNPs were primarily confirmed by change in colour from colourless to brown. UV-Vis spectrum of the AgNPs showed surface plasmon resonance (SPR) peak at 443 nm. Fourier transform infrared spectroscopy (FTIR) was used to key out the specific functional groups responsible for the reduction of silver nitrate to form silver nanoparticles and the capping agents present in the seed extract. The field emission scanning electron microscopy (FESEM) analyses revealed that the synthesized AgNPs were shape and the particle size was estimated in the range1-100 nm. The energy dispersive X-ray analysis (EDX) spectrum showed peaks for the presence in the range 5 Kev. The X-ray powder diffraction (XRD). The structural phase of the AgNPs was found in the form of face centred cubic (FCC).The antibacterial effect of nanoparticles produced Hygrophila Auriculata was studied using different pathogenic bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. From the disc diffusion methods, the synthesized silver nanoparticles showed an antibacterial activity.