Molecular variability in host specific isolate of Colletotrichum spp.
Abstract
Genus Colletotrichum attacking over 3,500 species of monocot and dicot plants. Genetic variability and multilocus DNA sequence analysis of 10 Colletotrichum spp. isolates from different host studied through gene marker (ITS 1-4, C- 28487- 23032, CT1-CT2B, ITS 4-5 primers). DNA sequence comparisons by three genes (ITS- 18S-26S, 28 rDNA and ITS- rDNA) verified species identity of C. truncatum, C. dematium, C. acutatum C. lindemuthianum, C. gloeosporioides and identity C. capsici. The correlation between morphological and molecular-based clustering demonstrated the genetic relationships among the isolates and species of Colletotrichum and indicated that ITS rDNA sequence data were potentially useful in taxonomic species determination.
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