Regeneration of Taxus baccata through vegetative propagation using different concentrations of IBA and NAA

Abstract

Taxus baccata is a highly important medicinal plant commonly known as ‘thuner” in Uttarakhand. The present research was accomplished to investigate the effect of different concentration of IBA and NAA on varying diameter in stem cuttings of Taxus baccata under different conditions for multiplication. The experimental work was performed at Ravigram Joshimath Chamoli under two environmental conditions in net house and in open nursery. Stems were cut into an equal length of 6 inches and divided into two varying size of diameter 0-4mm and 4-8mm from the field at the same day of treatment. Cuttings were pretreated for 24 hours in different concentration of IBA and NAA (100, 300, and 500ppm). After pretreatment of 24 hours these were transferred to the polythene bags containing Soil + vermicompost + Farm-yard manure at a ratio of 2:1:1. Proper irrigation was provided as per requirement of planted cuttings in rooting media. The highest survival percentage (58.25%) was obtained from IBA 500ppm followed by NAA100ppm. The Maximum fresh weight (27.237gm) was resulted from IBA 500ppm and dry weight (2.719gm) obtained from NAA100ppm. The study revealed that IBA 500PPM is the most optimum hormone for rooting percentage, fresh weight and dry weight of rooted stem cuttings of Taxus baccata.