De novo organogenesis from leaf explants in Piper longum L.

Abstract

De novo regeneration pathway from leaf callus was established in Piper longum L. Within three weeks of culture, leaf explants showed the callogenic response in media supplemented with various concentrations of TDZ. However no callus observed growth regulator free media. The callogenic response found highest (60%) in media supplemented with 0.5 mg l-1 TDZ.However, the per cent callus showing shoot bud (organogenic callus) was maximum (46.0%) in MS media supplemented with 0.25 mg l-1 TDZ which also showed maximum number (eight) of shoot bud per callus clump. These organogenic callus clumps when placed in MS + 1.5 mg l-1 BAP gave average 3-4 elongated shoots in each culture. The in vitro raised plants were successfully rooted in MS + 1.0 mg l-1 IBA and rooted shoots were subsequently hardened in Soil: Sand: FYM (1:1:1) with 70% success.