Identification of antioxidant compound from Phoenix pusilla fruits using NMR techniques

Abstract

The present investigation has been carried out to find the Antioxidant compound present in Phoenix pusilla fruits extract. Antioxidant compound was isolated by column chromatography technique. The collected flavonoid fractions was purified by thin layer chromatography. NMR studies was carried out to find the structure of Antioxidant compound Quercetin. 1H- NMR and 13C – NMR that reveals the structure of flavonoids. The compound was identified as 3, 3’, 4’, 5, 7 – pentahydroxyflavanone by 1H- NMR and 13C – NMR. All these data obtained in the present investigation supported the antioxidant compound present in Phoenix pusilla fruits extract and thereby traditional claim associated with Phoenix pusilla fruits.