Supplement of carbon and nitrogen nutrition towards assessment of biomass and virulence of rice fungal pathogen and endophytes

Abstract

Tweleve rice fungal pathogen namely Rhizoctonia solani, Curvularia lunata, Alternaria padwickii, Nigrospora oryzae, Sclerotium hydrophilum, Fusarium verticillioides, Fusarium sp, Rhizopus sp, Choanephora cucurbitarum, Acremonium sp, SM 1 and SM 2 were isolated from Swarna cultivar (MTU-7029) of rice at Jaguli instructional farm, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia district of West Bengal. In the present research, experiments were done to evaluate the impact of two different carbon (4 g/l and 8 g/l glucose) and nitrogen (4 g/l and 8 g/l peptone) sources along with control treatment (PD broth). The impact was correlated with the dry mycelial weight of the isolated rice fungal pathogen as well as virulence of the pathogen. Although the fungus could grow in all the carbon and nitrogen sources used here, but the momentum of growth as supported by the dry mycelial weight in different experiments, were not equal. Among all isolated twelve fungal pathogens of rice six (Rhizoctonia solani, Rhizopus sp., Choanephora cucurbitarum, Acremonium sp., SM-1 and SM-2) showed maximum biomass against carbon source and remaining six fungus (Sclerotium hydrophilum, Alternaria padwickii, Nigrospora oryzae, Curvularia lunata, Fusarium verticillioides and Fusarium sp.) showed maximum mycelial growth on nitrogen source. Hence, these nutritional behaviours of pathogen may be utilized for their sustainable management.