Evaluation of antioxidant potential of Pithecellobium dulce fruit against fipronil induced toxicity in rats

Abstract

Oxidative stress is considered as a biochemical event playing crucial role in the upset of the oxidant/antioxidant balance in the sense of exceeding the antioxidant defense capacity. The present investigation was carried out to evaluate antioxidative effect of the fruit extract of Pithecellobium dulce (Jungle jalebi) (300mg/kg B.w.) against oxidative stress induced by sub-acute exposure of Fipronil (10mg/kg B.w.) in rats. Sub-acute oxidative stress was conducted in adult male wistar rats. Rats were randomly divided into four groups (6 rats/group).Group I served as control in which corn oil (acting as a vehicle of Fipronil) was administered @10ml/kg B.w.Group II served as Fipronil treated group @10 mg/kg B.w. In Group III Fipronil along with Pithecellobium dulce fruits extract @300 mg/kg B.w. was administered and in Group IV Pithecellobium dulce fruits extract @300mg/kg B.w. was administered.Vehicle, Fipronil and Pithecellobium dulce were administered daily to the rats orally by gavage for 28 days. The dose of fipronil was selected on the basis of LD50 in rats. Lipid peroxidation, reduced GSH, SOD, catalase and glutathione reductase levels were estimated. Fipronil produced toxicity in the form of enhanced lipid peroxidation and reduced GSH, SOD and catalase levels. Pithecellobium dulce was significantly effective in restoration of these parameters towards normal. The study suggested that Pithecellobium dulce has ameliorating potential on oxidative stress following subacute exposure to fipronil in rats.