Molecular characterization of seed longevity and associated characters using SSR markers in soybean [Glycine max (L.) Merrill]

Abstract

The soybean is economically the most important oilseed in the world, unfortunately have poor competence for seed longevity. This study include set of 21 soybean genotypes grouped into good and poor seed longevity were evaluated phenotypically and same were analysed by 15 SSR marker with53.33% polymorphism. The marker Satt 281 gave distinct banding pattern by produced an amplicon size of 184 bp for those having poor seed longevity with higher electrolyte conductivity of the seed leachet and seed coat permeability across the genotypes, on the contrary other amplicon of size 235bp was produced for good seed longevity genotypes with electrolyte conductivity and seed coat permeability. This distinct banding pattern and genetic similarity coefficients obtained through SSR data analysis grouped the genotypes into two major clusters representing the good and poor seed longevity genotypes gave a clear cut differentiation between the two extreme of seed longevity at the molecular level.