Biochemical and molecular characterization of different Abrus precatorius L. genotypes varying in seed coat colour

Abstract

The aim of the present study intended mainly to investigate an interrelationship among three varieties of Abrus precatorius L. Varying in seed coat colour i.e. red, white and black. Biochemical study includes seed protein profiling by SDS PAGE and three isoenzymes (Catalase, Peroxidase and Superoxide dismutase) pattern by native PAGE. Molecular study carried out by RAPD. There were ten primers used, of which six were reproducible and significant. In protein profiling study total of 38 polypeptide bands were recorded ranging in molecular weight from 15.00 kDa to 95.00 kDa. Out of these 38 polypeptide bands, 23 were common among all three genotypes and 5 bands were polymorphic. Dendrogram of RAPD shows that red and white varieties have more similarities and grouped into the same cluster and the black variety was somewhat differ and grouped into the second cluster.